The need to verify of positive Mycoplasma hominis results obtained using the Mycoplasma ist 2 tests

The study draws attention to the possibility of falsely positive and negative results obtained by commercial test for detection of Mycoplasma hominis in clinical samples. Material/Methods: 5,715 samples were compared using the Mycoplasma IST 2 kit (BioMerieux) with conventional culture on PPLO (pleuropneumonia like organism) media. The discordant results were verified by PCR method using primers specific for 16S rRNA for M. hominis. Result: Due to bacterial and/or fungal flora overgrowth in 190 samples, the results for M. hominis were impossible to read and these specimens were excluded for analysis. The correlation of the two methods showed compliance for 5341 out of 5525 samples analyzed. Of 240 discordant results, (positive by the the Mycoplasma IST 2 kit) only 26,6% of these samples the presence of M. hominis was confirmed by growing on PPLO media and PCR assay. The sensitivity and specificity of the Mycoplasma IST 2 test as compared to the culture method as a “gold standard” were determined as 91% and 96%, respectively, while the positive and negative predictive value of the commercial test was 27% and 99%, respectively. Conclusions: In view of positive results obtained by the Mycoplasma IST 2 kit for M.hominis should be verified by other diagnostic methods.